Ma kēia ʻatikala, e wehewehe kikoʻī mākou i ke ʻano o ka ʻōnaehana TFF (Tangential Flow Filtration) a me ka mea e hoʻohana ʻia ai, e hāʻawi ana i kahi hiʻohiʻona o kāna mau noi, nā pono, nā pilikia a me ka wā e hiki mai ana.
Hoʻolauna i nā ʻōnaehana TFF
Tangential Flow Filtration is an advanced technique used to separate and purify biomolecules, such as proteins, cells and other biological components. Unlike direct filtration, where the flow passes perpendicularly through the filter, in TFF the flow passes tangentially to the membrane surface. This approach allows for a more kaʻina kānana kūpono, e hoʻemi ana i ke kūkulu ʻana o nā ʻāpana ma ka membrane a hoʻolōʻihi i kona ola.
ʻOi aku ka maikaʻi o ka ʻōnaehana TFF i nā noi kahi e koʻikoʻi ai ka mālama ʻana i ka pono o nā biomolecules a me kahi e pono ai ka hoʻokaʻawale kiʻekiʻe. Hoʻohana ʻia ia i loko o nā hale hana noiʻi a me nā hana ʻoihana.
Ka mōʻaukala a me ka ulu ʻana o TFF
Ua hoʻokumu mua ʻia ka kānana kahe wai i nā 1970s a me 1980s, ka mea nui no ka hoʻomaʻemaʻe protein a me ka hoʻokaʻawale ʻana i nā cell i loko o nā keʻena noiʻi. Mai ia manawa, ua ulu nui ka TFF a ua hoʻohui ʻia i nā kaʻina hana nui.
Ma mua, ʻoi aku ka maikaʻi o nā membrane i hoʻohana ʻia ma TFF a ʻoi aku ka maikaʻi o ka hoʻoheheʻe ʻana. Eia nō naʻe, me ka holomua o ka ʻepekema waiwai a me ka ʻenekinia membrane, ua hoʻomohala ʻia nā membrane ʻoi aku ka lōʻihi a maikaʻi. ʻO kēia mau holomua ka mea i hiki ai i nā ʻōnaehana TFF ke lilo i mea hana koʻikoʻi no ka hoʻomaʻemaʻe a me ka noʻonoʻo ʻana o nā huahana olaola, e hāʻawi ana i ka hana nui o nā lāʻau olaola a me nā huahana biotechnological ʻē aʻe.
Nā ʻano o nā ʻōnaehana TFF
Hoʻolālā ʻia kekahi mau ʻano ʻōnaehana TFF no nā noi like ʻole a me nā pono
Pehea e hana ai ka ʻōnaehana TFF?
I loko o kahi ʻōnaehana TFF, hoʻoheheʻe ʻia ka hopena ma ka ili o kahi membrane kānana. Mālama kēia kahe tangential i nā ʻāpana i ka neʻe ʻana, e hōʻemi ana i ke kūkulu ʻana i ka membrane a hoʻomaikaʻi i ka pono kānana. Hiki ke kaʻina hana sumi hoʻopaʻa ʻia ma nā pae aʻe:
- Hoʻomoe ʻai: Hoʻomākaukau ʻia ka hoʻonā i loaʻa i nā biomolecules a i ʻole nā palapala e hoʻokaʻawale ʻia a waiho ʻia i loko o ka ʻōnaehana TFF.
- ʻO ka paila a me ka kānana: Hoʻopili ʻia ka hopena ma o ka module membrane, kahi e mālama ai ke kahe tangential i nā ʻāpana i ka neʻe ʻana a pale i ka pani ʻana o ka membrane.
- ʻO ka hui paʻa a me ka hoʻopaʻa ʻana: ʻOhi ʻia ka filtrate (permeate) i loko o ka moku permeate, aʻo nā ʻāpana i hoʻokaʻawale ʻia (retainer) e hōʻiliʻili ʻia i loko o ka pahu hoʻopaʻa no ka hana hou ʻana a i ʻole ka hoʻopau ʻana.
Nā noi o nā ʻōnaehana TFF
- ʻO kaʻoihana olaki: ka hoʻomaʻemaʻe ʻana a me ka neʻe ʻana o nā protein, nā antibodies a me nā huahana olaola ʻē aʻe. Pono ka TFF i ka hana ʻana i nā lāʻau olaola, e like me nā lāʻau lapaʻau a me nā lāʻau lapaʻau monoclonal, e hōʻoiaʻiʻo ana i ka maʻemaʻe kūpono a me ka noʻonoʻo ʻana o nā huahana hope.
- ʻO Biotechnology: Ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana i nā cell, virus a me nā mea ola ʻē aʻe. Ma ka noiʻi biotechnological, hoʻohana ʻia ʻo TFF e hoʻomākaukau i nā laʻana olaola maʻemaʻe a paʻa no nā haʻawina like ʻole a me nā noi.
- ai hana: Ka nui o ka wai, nā meaʻai waiu a me nā meaʻai wai ʻē aʻe. ʻO nā ʻōnaehana kānana kahe e kōkua i ka hoʻomaikaʻi ʻana i ka maikaʻi a me ka paʻa o nā huahana meaʻai ma o ka wehe ʻana i nā haumia a me ka noʻonoʻo ʻana i nā mea i makemake ʻia.
- Lapaʻau wai: Hoʻomaʻemaʻe a hoʻomaʻemaʻe i ka wai ʻino a me ka wai inu. Hoʻohana ʻia ʻo TFF e wehe i nā mea haumia a me nā ʻāpana makemake ʻole mai ka wai, e hoʻomaikaʻi i kona ʻano maikaʻi a kūpono ia no ka hoʻohana hou ʻanasumption.
Nā ʻano kānana
I loko o ke kahua o ka ʻenehana kānana, aia kekahi mau ʻano i hoʻohana ʻia ma muli o ka nui o nā ʻāpana e hoʻokaʻawale ʻia a me ka noi kikoʻī:
Panina
In summary, tangential flow filtration (TFF) kahi ʻenehana holomua e hiki ai i ka hana pono ka hookaawale ana a me ka hoomaemae ana nā biomolecules a me nā ʻāpana. Ma muli o kāna hoʻolālā ʻana a me kāna hana, ua lilo ka kānana kahe kahe i mea pono i ka biotechnology hou a me nā ʻoihana like ʻole. ʻO ka hiki ke mālama i ke kahe mau a me ka hoʻomalu ʻia, me ka hōʻemi ʻana i ka pilikia o ka hoʻopaʻa ʻana, hoʻolilo i nā ʻōnaehana TFF ke koho i makemake ʻia no nā noi ʻoihana he nui.
Inā makemake ʻoe i ka honua o ka biotechnology, Hoʻokaʻaʻike TECNIC hui no ka ʻōlelo aʻo a me ke kōkua i ke koho ʻana mai kā mākou laulā o nā lako TFF, me nā wahi kānana mai 0.1m² a i 36.3m²
Nā nīnau i nīnau pinepineʻia (FAQ)
A Tangential Flow Filtration (TFF) system is an advanced technique used to separate and purify biomolecules such as proteins, cells and other biological components. Instead of flowing perpendicularly through the filter, in TFF the flow passes tangentially to the membrane surface.
I loko o kahi ʻōnaehana TFF, hoʻoheheʻe ʻia ka hopena ma ka ili o kahi membrane kānana. Mālama kēia kahe tangential i nā ʻāpana i ka neʻe ʻana, e hōʻemi ana i ke kūkulu ʻana i ka membrane a hoʻomaikaʻi i ka pono kānana.
Ma muli o ka nui o nā ʻāpana e hoʻokaʻawale ʻia a me ka noi kikoʻī, aia kekahi mau ʻano o ka kānana, me ka microfiltration, ultrafiltration a me nanofiltration, kēlā me kēia mea kūpono no ka hoʻokaʻawale ʻokoʻa a me nā pono hoʻomaʻemaʻe.
ʻO ka kānana Crossflow kahi inoa ʻē aʻe no ka kānana kahe tangential (TFF). Hāʻawi kēia ʻano hana i ka kānana ʻoi aku ka maikaʻi ma ka mālama ʻana i nā ʻāpana i ka neʻe ʻana a me ka hōʻemi ʻana i ke kūkulu ʻana ma ka membrane.
ʻO kekahi mau pilikia e pili ana i ka hoʻoponopono ʻana i nā kūlana kaʻina no nā ʻano biomolecules like ʻole, ka mālama ʻana i ka pono o ka membrane, a me ka mālama ʻana i nā kumukūʻai hana a mālama ʻia o ka ʻōnaehana.
