Chithandizo cha ma gene ndi kupita patsogolo kwa chithandizo cha matenda amtundu

Maselo a m'badwo wotsatira ndi mankhwala a majini (CGTs) akupereka chithandizo chochiritsira chomwe sichinachitikepo m'mbuyomu kwa odwala omwe akukumana ndi njira zochepa zochizira m'mikhalidwe inayake yazachipatala. M'nkhani ino, mankhwala othandizira imadziwika kuti ndi imodzi mwazinthu zodalirika kwambiri mu sayansi ya zamankhwala, zomwe zikusintha momwe matenda ena obadwa nawo amachitidwira komanso kuchiritsika. Zoyerekeza zikuwonetsa dziko lapansi Compound Annual Growth Rate (CAGR) ya 39.42% pakati pa 2022 ndi 2030, kutsimikizira mayendedwe othamanga komanso kuthekera kwakukulu mkati mwa gawo losinthali.

Zofunikira za Gene Therapy ndizo mavairasi (monga adenoviruses, lentiviruses, ndi retroviruses) kapena liposomes ndi njira zoperekera zachibadwa zomwe zingathe kupangidwa mwaluso (mwachitsanzo, pogwiritsa ntchito Crispr Cas9) kuti apereke majini opangidwa kapena mamolekyu osinthidwa a RNA ku maselo enieni a wodwalayo. Tekinolojeyi ili ndi njira zambiri zomwe zingagwiritsidwe ntchito, monga chithandizo cha matenda obadwa nawo, matenda obadwa nawo komanso matenda obadwa nawo, khansa komanso matenda ena opatsirana, monga momwe zingathere kusintha, kumanganso kapena kuzimitsa mitundu ina ya jini, potero. kupatsa maselo malangizo omveka bwino oti apange mapuloteni ofunikira kwambiri polimbana ndi matenda enaake. 

TECNIC teknoloji yowonjezereka mbiri ndiyoyenera upstream kupanga ndi zam'mbuyo downstream kuyeretsedwa kwa ma vector a virus omwe amafunikira. Timalola kukonza-kumapeto kwa magulu a virus pa labu, oyendetsa ndege ndi masikelo a mafakitale, kuchokera ku Research ndi Lab kupita ku Pilot ndi Pharmaceutical Industrial Production.

Kodi mankhwala othandizira majini ndi otani?

Malinga ndi Food and Drug Administration (FDA), chithandizo cha majini chimatanthauzidwa ngati "Zomwe zimayenderana ndi zotsatira zake polemba ndi/kapena kumasulira kwa chibadwa chosinthidwa ndi/kapena kuphatikizika ndi ma genome omwe amaperekedwa ngati ma nucleic acid, ma virus, kapena ma genetic engineering microorganisms". Chifukwa cha kulondola kwake, sayansi yasayansi iyi yapangidwa kuti ikonze zomwe zimayambitsa matenda.

Yakhala imodzi mwa madera omwe akukula mwachangu komanso odalirika mumankhwala obwezeretsanso, ndi ntchito zosiyanasiyana monga gene therapy khansa. Kukula kwake kumafikira kuchiza matenda otengera chibadwa, khansa ndi matenda opatsirana, kuwonetsa kuthekera kwakukulu kothandizira kusintha kwachipatala. Njira yapakati pamankhwala a jini imayenderana ndi kugwiritsa ntchito vekitala kuti alowetse jini mwachindunji m'maselo a wodwala (mu vivo) kapena mosadukiza kudzera m'maselo asanayambe kulowetsedwa mwa wodwala (ex vivo). Cholinga chake ndi kukonzanso kapena kusintha mtundu wina woyipa, kuzimitsa jini yosokonekera, kapena kuyambitsa majini atsopano kuti achire ndi kuchiza matenda obwera chifukwa cha kusintha kwa majini., kutsegulira njira yolondola komanso yolunjika pazamankhwala azachipatala.

Ubwino wa mankhwalawa ndikuti amatsimikizira kuperekedwa kwa "chithandizo choyenera pa mlingo ndi nthawi yoyenera", poganizira mayankho amunthu pamankhwala ndi ma chibadwa awo. Mosiyana ndi mankhwala achikhalidwe, omwe nthawi zambiri amafunikira kuwongolera pafupipafupi komanso kuyang'ana kwambiri pakuwongolera zizindikiro ndi kukula kwa matenda, gene therapy ikufuna kuthana ndi jini lomwe silikugwira ntchito ndikupereka chithandizo chanthawi yayitali. ndi mlingo umodzi wokha poyang'ana chomwe chimayambitsa matenda obadwa nawo.

Kutengera ndi mitundu ya ma cell omwe amathandizidwa, pali mitundu iwiri ya chithandizo cha majini:

Somatic Therapy

Germline Therapy

Mavuto mu gene therapy

Kuzindikira machiritso apamwamba omwe angakhalepo kuchiza matenda osiyanasiyana otengera chibadwa, pakufunika kwambiri kuthetsa kufunikira kowonjezereka kwa mankhwala a chibadwa. Kuchokera pamalingaliro a biotech, kupereka jini yochizira kumalo enieni, kuyimitsa ndikupewa kuyankha kwa chitetezo chamthupi ndizovuta kale. Komabe, kupanga njira zochiritsira zamtunduwu zimakumananso ndi zovuta zaposachedwa komanso zanthawi yayitali ndikuganizira momwe angapangire mitundu iyi yamankhwala kukhala yowopsa, yocheperako nthawi.suming ndi yotsika mtengo akadali chopinga chopitirira chomwe sichiyenera kugonjetsedwa.

Zovuta za scalability
Makhalidwe ofunika kwambiri
Mtengo wa chithandizo cha majini

Advanced Bioreactors

Kupanga ma viral vector kumagwiritsa ntchito njira zovuta zomwe zimabweretsa zovuta zosiyanasiyana munthawi yonse ya moyo wazinthu. Poyankha mavutowa, TECNIC zachitika autozida za matic bioprocess kwa onse awiri upstream ndi downstream Chitukuko. Timamvetsetsa kuti, mosiyana ndi zopanga zamaukadaulo zamasayansi (monga mamAbs ndi ma biologics ena), kupanga ndi kukonza machiritsowa kumafuna kuti zida za m'manja zizikhalabe ndi mikhalidwe yofunikira (CQAs) munthawi yonseyi.

Chifukwa chake, ngati cholumikizira chamtundu uliwonse wamankhwala ndi vekitala, makampani amayenera kusungitsa ma vector odalirika kuti apange. Chifukwa chake, TECNIC eLAB, ePILOT ndi ePROD bioreactors ⇀ ndi zokonzedwa kuti zitsimikizire zotsatira zapamwamba komanso zopangika kuthana ndi zovuta zomwe tazitchula kale. Timapereka yankho labwino kwambiri la bioprocess pazamankhwala amtundu, popeza zida zathu zidapangidwa mwaluso kuti zizitha kuyang'anira momwe chilengedwe chimakhalira, kukhathamiritsa ma cell kuti apeze zokolola zambiri ndikuchepetsa zothandizira komanso kugwiritsa ntchito nthawi. 

Kuphatikiza apo, zida zathu zidapangidwa ndi automatic unit control system yomwe imaphatikizapo Integrated kachitidwe ka data analytics, zomwe zimakuthandizani kuti musinthe bwino chikhalidwe kuti mukhale ndi ntchito yosalala ya bioprocess kuti muwonjezere kupanga kwanu. Komanso, TECNIC's scalability chibadidwe ndi kusinthasintha kwa zida zathu zimatsimikizira a bwino makulitsidwe-mmwamba ndondomeko ndi automatic control system ndi ma aeration oyenerera ndi ma agitation system. Kaya mukuchita upainiya kapena mukuyendetsa ntchito yopanga mafakitale, tithandizireni kukwaniritsa zomwe mukufuna. 

Advanced Bioreactor Solutions ⇀

Tangential Flow Filtration mu gene therapy

Zambiri mwa kafukufuku ndi chitukuko cha bioprocesses of cell and gene therapies (CGTs) ayang'ana kwambiri pakukula kwa upstream ndondomeko. Ngakhale R&D ikuyenera kupitiliza kuyang'ana kwambiri pakukulitsa kachulukidwe ka cell ndi zosowa zina zakukulira kwa ma cell, zolepheretsa kupanga zikutsamira. downstream kukonza. Kuti tikwaniritse chosowachi mokhazikika kwambiri komanso kutulutsa kwakukulu, athu eLAB TFF ⇀, eLAB TFF SU ⇀, ePILOT TFF ⇀ ndi ePROD TFF ⇀ perekani yankho lomwe likhoza kukulitsidwa, autozogwirika, zopezeka mokwanira komanso zothekera kuchita ntchito zachitukuko. Kaya mukuchita upainiya kapena mukuyendetsa ntchito yopanga mafakitale, tithandizireni kukwaniritsa zomwe mukufuna kuti mupange mapulojekiti anu othandizira ma cell.

Kuonetsetsa kuti zikupita patsogolo upstream processing akuchulukitsidwa, TECNIC zida zimatsimikiziranso zogwira mtima downstream chitukuko cha ndondomeko pakuchepetsa kuchuluka kwa ma virus, kukolola ma virus komanso kuyeretsa kangapo ndi njira zosinthira ma buffer. Pa nthawi yomweyo, a autokukonza zida zathucipes imachepetsa kwambiri kufunika kosintha pamanja, kukulitsa magwiridwe antchito komanso kuchepetsa ndalama zogwirira ntchito. Kuphatikiza apo, timakuthandizani kuti mukhale ndi chilolezo chokhala ndi mapuloteni ambiri komanso kuchira kwambiri kwa ma cell omwe amatha kukhala ndi mphamvu zochepa pakukula kwawo. Kupereka mayankho odalirika a kusefera ophatikizidwa ndi kuthamanga kwambiri komanso kusungika kwakukulu kwa solute, mawonekedwe athu apamwamba amapangidwa kuti akhale abwino kwambiri. downstream kukonza ma vectors. Komanso, wathu patsogolo automachitidwe a matic amapereka a wogwiritsa ntchito bwino pamasikelo osiyanasiyana ogwiritsira ntchito, kuwonetsetsa kuti zotsatira zake zimakhala zosasinthasintha komanso zosasinthasintha.

Nkhani zopambana ndi kugwiritsa ntchito

Poganizira zovuta za bioprocess, sizodabwitsa kuti mtengo wodziwika bwino wamankhwalawa. At TECNIC, tikufuna kuthandiza makasitomala athu kuti apangitse chithandizo cha majini kuti chiwonjezeke, motero, kuti odwala azitha kupezeka mosavuta.. Monga ukadaulo wina uliwonse, mtengo wake watsika pakapita nthawi ndi luso labwino kwambiri, ndipo, ngakhale sitingathe kupereka mwatsatanetsatane chifukwa chachinsinsi, pakhala pali zochitika zambiri pomwe TECNICZida zathandizira kwambiri kupanga mankhwalawa.

Mwachitsanzo, m'modzi mwamakasitomala athu ndi kampani yaukadaulo yomwe ili pano kuphunzira kugwiritsa ntchito CRISPR CAS 9 kwa exon skipping therapy ya Duchenne muscular dystrophy. Mwa "kudumpha" magawo ena amtundu wa DNA pogwiritsa ntchito ukadaulo wa Crisp Cas, amatha kuchepetsa kufooka kwa minofu yamtunduwu. Pakusintha kwa In vivo, akugwiritsa ntchito ma virus opangidwa ndi athu kwathunthu automatic bioreactor, eLAB Advance ndipo, chifukwa cha masinthidwe ake osunthika, amatha kusintha chikhalidwecho malinga ndi zosowa za mayeso aliwonse. Mpaka pano, bioreactor yathu yawapatsa mphamvu kuti azitha kuchita bwino kwambiri ndikuwongolera mayesero osiyanasiyana osiyanasiyana, kuwapangitsa kuwongolera chikhalidwe chawo pogwiritsa ntchito automatic control unit yokhala ndi kuwongolera kwathunthu pazofunikira pamaphunziro awo.

Onani zida zathu zonse

Onani zamtsogolo za biothecnology ya m'badwo wotsatira wama cell and gene Therapies (CGTs) ndi TECNIC's zida. Ma bioreactor athu adapangidwa kuti azigwiritsidwa ntchito mosiyanasiyana, kuwonetsetsa kuti zikuyenda bwino komanso zabwino. Dziwani zambiri zamayankho athu asayansi yazachilengedwe, kuphatikiza ma Bioreactors, Tangential Flow Filtration system, ndi zina zambiri. Pitani patsamba lathu kuti mumve Ma bioreactors ⇀Kusefera kwa Tangential Flow ⇀ndipo Lumikizanani ndi ⇀ kuti apitirize information ndi kufunsa.