Kuwunika Kupanga Mapuloteni a Recombinant ndi TECNIC'S Biotech Solutions

Mu gawo lamphamvu la biotechnology, kupanga recombinant mapuloteni yakhala imodzi mwa njira zazikulu zopangira amapanga mapuloteni omwe amafunidwa kwambiri kwa mitundu yambiri ya ntchito za sayansi ya moyo, monga kafukufuku wamankhwala ndi mankhwala.

M'mbuyomu, njira yayikulu yopezera puloteni inayake inali kuyipatula kuzinthu zachilengedwe, zomwe nthawi zambiri sizinali zogwira ntchito komanso zotengera nthawi.sumndi. Komabe, chifukwa cha kupita patsogolo kwaposachedwa kwa uinjiniya wa majini ndi njira zopangira mamolekyu achilengedwe, mawonekedwe a biotech asintha, kupangitsa kuti zitheke. fotokozerani mapuloteni ophatikizana mkati mwa machitidwe odzipatulira, monga mabakiteriya, yisiti, maselo a tizilombo, algal system ndi maselo a mammalian.

izi luso la biotechnological zakhala zofunika kwambiri pa biomedicine ndi bioprocesses zamankhwala, komanso kuzinthu zina zaukadaulo waukadaulo, zomwe zimapereka njira zatsopano zothetsera zovuta zapadziko lonse lapansi. Pamene ukadaulo uwu ukupitilirabe kusinthika, kuthekera kwake kukulonjeza kupititsa patsogolo njira zathu zamagawo ambiri a sayansi ya moyo.

At Tecnic Timazindikira ntchito yofunika kwambiri yomwe mapuloteni ophatikizananso amagwira nawo mu sayansi ya zamankhwala, ndipo chifukwa chake, tikuwonetsetsa kuti zida zathu zamakono zidapangidwa mwaluso kuti zikweze ndi kukhathamiritsa kupanga kwake. Mwachangu, molondola ndi zovuta. Mitundu yathu yonse yama bioreactors imapatsa mphamvu ofufuza ndi opanga mafakitale kuti akwaniritse zokolola zowonjezera, ndi machitidwe athu a TFF amalola zosavuta komanso autokuyeretsedwa kwa mamatic wa chinthu chomwe mukufuna.

Onani zotheka zopanda malire ndi Tecnic ndikusankha kuti tigwire ntchito ndi mnzake wodzipereka kuti tithandizire pakupanga bwino pogwiritsa ntchito njira zathu zatsopano.

Kodi ma recombinant protein ndi chiyani?

Ngati mapuloteni ali ochuluka kwambiri muzinthu zachilengedwe, kuyendetsa ma cell bioprocesses, recombinant mapuloteni ndi amenewo amapangidwa kuchokera ku jini yophatikizanso ya DNA, zomwe zakhala zopangidwa ndi chibadwa kupangidwa kukhala a vesi vesi. Kamodzi analowetsedwa mu chamoyo cholandira, vekitala imathandizira kufotokoza kwa mapuloteni ophatikizananso kudzera m'mawu ndi kumasulira kotsatira kwa RNA m'maselo omwe amafunidwa.

The chamoyo cholandira (mwachitsanzo E. coli), tsopano chibadwa kusinthidwa, kwenikweni amakhala fakitale apadera kwa kupanga recombinant protein. Njira ya sayansi ya zamoyo imeneyi ndi yothandiza kwambiri, ikuthandiza kupanga mapuloteni omwe angakhale ovuta kuwapeza ochuluka kapena kusinthidwa mwatsatanetsatane pogwiritsa ntchito njira zachikhalidwe. Mapuloteni ophatikizana amabweretsa phindu lalikulu ku sayansi ya moyo, chifukwa amalola kupanga zinthu zama protein zomwe zimafunidwa kwambiri zomwe zimakhala ndi zokolola zambiri pazopanga zazikulu

Komanso, by pogwiritsa ntchito njira zama genetic engineering kusintha DNA ndondomeko kabisidwe puloteni inayake, n'zotheka kusintha kusintha mu kapangidwe kake, ntchito, kapena zinthu zina zobadwa nazo, zomwe zimagwira ntchito ngati njira injiniya recombinant mapuloteni wokhala ndi mawonekedwe ogwirizana kuti akwaniritse zofunikira zosiyanasiyana. Kusinthika kwachilengedwe kumeneku pakupanga mapuloteni kumapitilira kupitilira kupanga mapuloteni atsopano pazifukwa zosiyanasiyana, komanso kumathandizirapassndi kuwonjezera zomwe zilipo.

Recombinant protein imagwiritsa ntchito

Pali mitundu ingapo ya mapuloteni ophatikizananso, iliyonse ili ndi magawo ake ogwiritsira ntchito, koma ambiri a iwo akhala ofunikira m'njira zosiyanasiyana. madera azachipatala.

Mwachitsanzo, mu makampani opanga mankhwala, kupanga ma recombinant mapuloteni ndikofunikira kwambiri kupanga katemera ndi mankhwala, chifukwa nthawi zambiri amagwira ntchito ngati mankhwala opangira mankhwala mankhwala. Mwachitsanzo, ma antibodies ophatikizananso monga ma antibodies a monoclonal kapena ma immunoglobulin antibodies ndi njira zina zochiritsira zotsogola nthawi zambiri amapangidwa pogwiritsa ntchito njira yowonetsera puloteni iyi. Komanso, kulondola komanso kusinthika kwaukadaulo waukadaulo wophatikizanso mapuloteni kwasintha chitukuko cha mankhwala, zomwe zimathandiza kupanga mapuloteni apamwamba, ovomerezeka ochizira monga insulini, zinthu zomwe zimakula kapena mahomoni ena.

M'munda wa diagnostics, pamene ntchito ndi kupezeka kwa mapuloteni ophatikizananso akuwonjezeka, amakhala amagwiritsidwa ntchito popanga mayeso ozindikira kwambiri komanso apadera kwa matenda osiyanasiyana. Mwachitsanzo, ngakhale kachilomboka sikungathe kukulitsidwa, malinga ndi mndandanda wa jini, ndizotheka kuyankha mwachangu ma virus omwe akubwera kapena ma virus atsopano ndi mayeso a ELISA omwe amapangidwa pogwiritsa ntchito mapuloteni ophatikizananso a kachilomboka.

 

Udindo wofunikira pakufufuza

In kafukufuku, zakhala zofunikira kwambiri kuphunzira za ntchito ya mapuloteni ndi kuyanjana, zomwe ndizofunikira kuti timvetsetse njira zamoyo ndi njira za matenda. Kuphatikiza apo, njira zotsogola monga ma plasmon resonance amagwiritsidwa ntchito kuwunika momwe amagwirira ntchito, zomwe zimathandiza kumvetsetsa mozama za ntchito zawo zachilengedwe komanso kuzindikira momwe mapuloteniwa amagwirira ntchito. Mwachitsanzo, kufotokoza mapuloteni mu E. coli walola kafukufuku wa zamankhwala kuti aphunzire njira zovuta za biochemical ndi decipIye ndi mamolekyu omwe amayendetsa zinthu zosiyanasiyana zamoyo. Kuphatikiza apo, pogwiritsa ntchito zida zosinthira ma genome monga CRISPR CAS9 kapena ukadaulo wa Cre-lox, kugwiritsa ntchito mapuloteni ophatikizananso amitundu monga Rag 1 ndi Rag 2 kapena jini ya CNTF kwapereka chidziwitso chofunikira pakuwongolera mawonekedwe a jini, njira zosinthira ma sign, ndi kuyanjana kodabwitsa pakati pa zigawo zosiyanasiyana zama cell.

Kuphatikiza apo, mapuloteni ophatikizananso omwe amagwiritsidwa ntchito muukadaulo siwofunikira pazamankhwala komanso magawo ena monga ulimi ndiukadaulo watsopano wazakudya, kupereka mayankho odalirika ku zovuta zovuta m'makampani a biotech. Pamene ukadaulo uwu ukupitilirabe kusinthika, kuthekera kwake kwazatsopano zamtsogolo kukulonjeza kusinthiratu njira yathu ya sayansi ya moyo, pofufuza ndi kugwiritsa ntchito mafakitale, komanso TECNIC tikufuna kukhala gawo la njira zatsopano zopititsira patsogolo kusanduka pamodzi.

Zovuta pakupanga mapuloteni ophatikizana

Biotechnology iyi imafunikira zida zambiri komanso luso, ndipo kulephera pakupanga kumatha kukhala ndi zotulukapo zazikulu komanso zovuta zachuma. Mavuto ake wamba ecompass nkhani zokhuza kuchita bwino, kuyeretsedwa ndi scalability ndipo amafuna kuganiziridwa mosamalitsa ndi mayankho apamwamba kuti pakhale zotsatira zabwino.

Kuchita bwino kukhathamiritsa zokolola pamene kuchepetsa chuma

Kuonetsetsa chiyero ndi kukhulupirika kwa ntchito

Kukonzekeletsa malo okhala kuti afotokoze bwino zama protein

Kuthana ndi zovuta za scalability

Kufunika kwa TFF Pakupanga Mapuloteni Ophatikizanso

Kuti apange mapuloteni opangidwanso oyeretsedwa kwambiri okhala ndi zokolola zambiri komanso zotsatira zosasinthika, ndikofunikira kugwira ntchito ndi zida zabwino kwambiri, kaya mukupanga ma antibodies ochizira pofuna kufufuza kapena kupanga ma enzymes akuluakulu amakampani. Monga yankho loyamba, TECNIC Ma bioreactors ⇀ kuyimilira upstream recombinant protein bioprocessing kupanga, kuthana ndi zovuta zazikulu ndi luso pa sitepe iliyonse, kuyambira kafukufuku mpaka kupanga makampani akuluakulu.

Tiloleni kuti tiziwongolera moyenera magawo onse a chilengedwe, kukhathamiritsa bwino ma cell kapena tizilombo tating'onoting'ono zokolola zomanga thupi ndikuchepetsa kugwiritsa ntchito nthawi. Kuthekera kwamphamvu kuwonetsetsa kuti bioprocess ndi yolondola kwambiri TECNIC automatic control unit of eLAB, ePILOT ndi ePROD ⇀ , yokhala ndi PLC yamakampani, imakuthandizani kuti musinthe chikhalidwe chanu kuti mukwaniritse zotsatira zobwerezabwereza komanso zosasinthika.

Gawo lomaliza la chikhalidwe likakwaniritsidwa, ndizofunikira kupeza a zotsatira zabwino zomaliza. Zotsogola downstream Kusefera kwa Tangential Flow kwa TECNIC ⇀ amalekanitsa bwino puloteni yomwe mukufuna kuchokera ku zonyansa ndi zowononga, kuonetsetsa kuti pali chiyero chapamwamba chokhala ndi maulendo othamanga kwambiri. Komanso, chifukwa chake kwathunthu automatic system ndi zosankha zosiyanasiyana zogwirira ntchito, eLAB TFF ⇀ , ePILOT TFF ⇀ ndi ePROD TFF ⇀ thandizirani ku chiyero chomaliza cha bioprocess yanu yonse.

Pomaliza, anapatsidwa scalability chibadidwe cha zida zathu, TECNICMa bioreactors ndi tangential system adaptability zimatsimikizira kudalirika komanso kuchita bwino komwe kumafunikira kuti zikwaniritse zosowa zamapuloteni apamwamba kwambiri. Chifukwa chake, kaya mukuchita upainiya kapena mukuyendetsa kupanga mafakitale, TECNICKudzipereka ndi njira yothetsera kusinthika pamodzi.

Nkhani zopambana ndi kugwiritsa ntchito

Ngakhale sitingathe kupereka zambiri chifukwa chachinsinsi, pakhala pali zochitika zambiri TECNICZida zathandizira kwambiri kupanga mapuloteni ophatikizananso. Makasitomala athu adayika chidaliro chawo mwa ife, ndi kupambana kwa zomwe zaperekedwa TECNIC mayankho amatsimikizira kupambana kwathu ndi kudzipereka kwathu kuti tisinthe limodzi.

Mwachitsanzo, mu gawo la kafukufuku watsopano chakudya, mmodzi wa makasitomala athu cholinga kutulutsa mapuloteni opangidwa ndi dzira kwambiri pogwiritsa ntchito gwero losakhala la nyama. Ngakhale anali kale ndi ukadaulo komanso chidziwitso, adakumana ndi zovuta zingapo panthawi yopanga Scale-Up. Kugwiritsa ntchito TECNIC eLAB Advanced Bioreactor, adatha kukhathamiritsa dongosolo la mawu, zomwe zimatsogolera ku a kuwonjezeka kwakukulu kwa zokolola zonse ndi chiyero. Komanso, kusinthasintha kwa zida izi, kuphatikiza ndi mawonekedwe ake, zimawalola kuti azigwira ntchito mosasunthika. zombo zambiri zamagawo osiyanasiyana akupanga. Zotsatira zake, kasitomala wathu adakwaniritsa zolinga zawo zopanga ndi kuwongolera bwino komanso mtundu wazinthu, ndikupeza zotsatira zabwino kwambiri pantchito yawo.

Kudzipereka kwathu kumapulojekiti amakasitomala kumatsimikizira kuti timakhala osinthika pazomwe zikuchitika kapena thandizo lina lililonse lomwe lingafunike, ndipo pakadali pano, tikuperekabe chithandizo chanthawi zonse ndikukhala otanganidwa ndi polojekitiyi.

M'gawo lazamankhwala, TECNIC amagwiritsidwa ntchito mogwirizana ndi makampani omwe ali imayang'ana kwambiri pakupanga kwakukulu kwa zinthu za biotech pharma monga Ma antibodies a monoclonal ⇀mankhwala oletsa khansa, ma cell mankhwala ⇀Ndipo kwambiri.

Izi zotsogola zamankhwala ndi biotechnology nthawi zambiri zimafuna kugwiritsa ntchito bioreactors chifukwa upstream ndondomeko ndi downstream tangential filtration systems kuonetsetsa zokolola zabwino, chiyero, ndi scalability.

Mwachitsanzo, TECNIC amakhudzidwa kwambiri ndi kupanga kwakukulu kwa mankhwala atsopano achire opangira ziweto. Pantchito yapaderayi, zida zathu za ePROD zimagwira ntchito yofunika kwambiri, kupereka kulondola komanso kuwongolera bwino kwa bioprocess kuti zitsimikizire zokolola zambiri zomwe zikuyembekezeka pulojekitiyi. Pambuyo pake, ePROD TFF yathu imalekanitsa bwino peptide yomwe mukufuna kutengera chikhalidwe cha media ndi zonyansa zina. Pakali pano, polojekiti yonse ikukwaniritsa a chiyero chapamwamba chophatikizana ndi zokolola zabwino, umboni wa kudzipereka kwathu pakupambana kwamakasitomala popereka mayankho abwino kwambiri pagawo lililonse la bioprocess.

Onani zida zathu zonse

Onani kutsogolo kwa mapuloteni ophatikizana ndi zida zathu zapamwamba. Ma bioreactor athu adapangidwa kuti azigwiritsidwa ntchito mosiyanasiyana, kuwonetsetsa kuti zikuyenda bwino komanso zabwino. Dziwani zambiri zamayankho athu asayansi yazachilengedwe, kuphatikiza ma Bioreactors, Tangential Flow Filtration system, ndi zina zambiri. Pitani pamasamba athu Ma bioreactors ⇀, Kusefera kwa Tangential Flow ⇀ndipo Lumikizanani ndi ⇀ kuti apitirize information ndi kufunsa.