Gene therapy uye kufambira mberi mukurapa kwezvirwere zvemajini

Inotevera-chizvarwa sero uye majini kurapwa (CGTs) vari kupa kurapa kusati kwamboitika kune varwere vanotarisana nemaitiro mashoma ekurapa mukati memamwe mamiriro ekurapa. Muchirevo ichi, kurapa kwemajini inomira pachena seimwe yeanonyanya kuvimbisa mukati mebiotechnology, kushandura mabatirwo echimwe chirwere chemajini uye chinogona kurapwa. Kufungidzira kunoratidza inofungidzirwa pasi rose Compound Annual Growth Rate (CAGR) ye39.42% pakati pa2022 na2030, ichisimbisa kukurumidza kukurumidza uye kugona kukuru mukati meiyi dura rekushandura.

Izvo zvakakosha zveGene Therapy ndizvo viral vectors (zvakadai seadenoviruses, lentiviruses, uye retroviruses) kana liposomes inzira inoshanda yekuendesa yemajini anogona kugadzirwa zvine hungwaru (somuenzaniso, kushandisa Crispr Cas9) kuendesa majini akagadzirwa kana mamorekuru akagadziridzwa eRNA kumasero chaiwo omurwere. Iyi tekinoroji ine huwandu hwakasiyana hwezvinogona kushandiswa, senge kurapwa kwezvakagara nhaka, nhaka yekuzvarwa chirwere uye genetic kusagadzikana, gomarara uye kunyangwe zvimwe zvirwere zvinotapukira, sezvazvinogona kugadzirisa, kuvaka patsva kana kudzima inobvisa gene musiyano, nekudaro. kupa masero nemirayiridzo chaiyo yekugadzira mapuroteni anobatsira akakosha mukurwisa zvimwe zvirwere. 

TECNIC scalable tekinoroji portfolio yakanyatsokodzera upstream kugadzirwa uye shure downstream kucheneswa kwemavhairasi mavhairasi anodiwa. Isu tinobvumira kupera-kusvika-kumagumo kugadzirisa kwehutachiona mabhechi palabhu, mutyairi uye zvikero zveindasitiri, kubva kuKutsvagisa uye Lab kuenda kuPilot uye Pharmaceutical Industrial Production.

Chii chinonzi gene therapy?

Maererano neChikafu neDrug Administration (FDA), kurapwa kwemajini kunotsanangurwa se "Zvigadzirwa zvinoyananisa mhedzisiro yazvo nekunyora uye/kana dudziro yezvakatamiswa genetic zvinhu uye/kana nekubatanidza mune inotambira genome uye inopihwa se nucleic acids, mavhairasi, kana genetically engineered microorganisms". Nekuda kwehunhu hwavo chaihwo, iyi biotechnology yakagadzirirwa kururamisa mudzi wechikonzero chechirwere chakapihwa.

Yakava imwe ye zvakanyanya kukurumidza kushanduka uye minda inovimbisa mune regenerative mushonga, nemashandisirwo akasiyana siyana senge gene therapy cancer. Chiyero chayo chinosvika pakurapa kwenhaka genetic kusagadzikana, gomarara uye zvirwere zvinotapukira, zvichiratidza kugona kukuru kwekuchinja kwekurapa kupindira. Iyo yepakati nzira mukurapa kwemajini inotenderera pakuisa vheji yehutachiona kuunza gene zvakananga mumaseru omurwere (in vivo) kana zvisina kunanga nekupinza maseru asati apinzwa mumurwere (ex vivo). Chinangwa ndechekugadzira patsva kana kushandura mutsauko wakashata, kudzima majini asina kukwana, kana kuunza majini matsva ekurapa nekurapa zvirwere zvinokonzerwa nekushandurwa kwemajini., kugadzira nzira yechokwadi uye yakanangwa biotherapeutic mhedzisiro.

Kubatsira kwemushonga uyu wakasarudzika ndewekuti unovimbisa kuendesa "kurapa kwakakodzera panguva chaiyo uye nenguva", tichifunga nezvemhinduro dzemunhu kumishonga uye mararamiro avo. Kusiyana nemishonga yechinyakare, iyo inowanzoda kutungamirirwa uye kuisa pfungwa pakugadzirisa zviratidzo uye kufambira mberi kwechirwere, gene therapy ine chinangwa chekugadzirisa geni risina kushanda uye kupa rubatsiro rwekugara kwenguva refu. pamwe chete nedosi rimwe chete nekunangana nechikonzero chinokonzera chirwere chemajini.

Zvichienderana nemhando dzemasero anorapwa, kune marudzi maviri akasiyana egene therapy:

Somatic Therapy

Germline Therapy

Matambudziko mukurapa gene

Kuziva nzira dzokurapa dzepamusoro dziripo kurapa zvirwere zvakasiyana-siyana zvakagarwa nhaka, pane kudiwa kukuru kwekugadzirisa kudiwa kuri kuwedzera kwemishonga yemajini. Kubva pakuona kwebiotech, kuendesa jini yekurapa kunzvimbo chaiyo, kuishandura uye kudzivirira mhinduro yekudzivirira kwezvirwere zvatove dambudziko. Zvakangodaro, iyo nzira yekugadzira yeaya genetic marapirwo anotarisanawo nekukurumidza uye kwenguva refu matambudziko uye kufunga maitiro ekuita aya marudzi ekurapa scalable, nguva shoma-con.suming uye inodhura-inoshanda ichiri chipingamupinyi chinopfuurira icho chisati chakundwa.

Scalability matambudziko
Hunhu hwakakosha
Mutengo we gene therapy

Advanced Bioreactors

Kugadzirwa kweViral vector kunoshandisa maitiro akaomarara izvo zvinoguma nekusiyana kwakasiyana mukati mehupenyu hwechigadzirwa. Mukupindura matambudziko aya, TECNIC zvave automatic bioprocess midziyo yezvose zviri zviviri upstream uye downstream budiriro. Isu tinonzwisisa kuti, kusiyana nekugadzirwa kwechinyakare biotech (senge maAbs uye mamwe biologics), kugadzirwa uye kugadzirisa kweaya marapirwo kunoda kuti cellular material ichengete yakakosha hunhu (CQAs) mukati mese maitiro.

Naizvozvo, kana iyo linchpin yeese gene therapy iri vector, makambani anofanirwa kuchengetedza yakavimbika vector kugadzirwa kwekugadzira kwayo. Nechikonzero ichocho, TECNIC eLAB, ePILOT uye ePROD bioreactors ⇀ ndivo yakagadzirirwa kuve nechokwadi chemhando yepamusoro uye inoberekazve mhedzisiro kukunda matambudziko ambotaurwa. Isu tinopa yakanakisa bioprocess mhinduro yejini marapirwo, sezvo zvishandiso zvedu zvakagadzirirwa zvine hungwaru kuti zvipe kutonga kwakaringana pamusoro pezvakatipoteredza paramita, optimize cellular mamiriro egoho rakawanda uku uchideredza zviwanikwa uye kushandisa nguva. 

Uyezve, midziyo yedu yakagadzirwa ne automatic unit control system inosanganisira yakabatanidzwa data analytics systems, iyo inokugonesa kukwenenzvera mamiriro etsika kuti ugadzire yakatsetseka bioprocess kuita kuitira kukwirisa yako yekugadzira bioprocess. Uyezve, TECNIC's inherent scalability uye kuchinjika kwemidziyo yedu inovimbisa a kubudirira kukwira-up maitiro with an automatic control system uye yakakodzera aeration uye agitation system. Kunyangwe iwe uri kuita tsvakiridzo yekupayona kana kutyaira maindasitiri-chikero kugadzirwa, tigonese kusangana nemagumo ekuda kwako. 

Yepamberi Bioreactor Solutions ⇀

Tangential Flow Filtration mune gene therapy

Yakawanda yekutsvagisa nekusimudzira bioprocesses yesero uye gene kurapwa (CGTs) vakatarisa pakusimba kwe upstream urongwa. Nepo R&D ichifanirwa kuenderera mberi ichitarisa mukuvandudza maseru uye zvimwe zvinodiwa zvekuwedzera masero, bhodhoro rekugadzira riri kutsamira kune downstream processing. Kuti tigadzirise chinodiwa ichi nehupamhi hwepamusoro uye hukuru hwepamusoro, yedu eLAB TFF ⇀, eLAB TFF SU ⇀, ePILOT TFF ⇀ uye ePROD TFF ⇀ kupa mhinduro inogona kuyerwa, autoyakabatana, yakazara uye inogoneka kugadzirisa zviitiko zvebudiriro. Kunyangwe iwe uri kuita tsvakiridzo yekupayona kana kutyaira maindasitiri-chikero kugadzirwa, tigonese kusangana nemagumo ako ekugadzira mapurojekiti ako ekurapa sero.

Kuti ave nechokwadi chekuti kufambira mberi mukati upstream processing inowedzerwa, TECNIC midziyo inovimbisawo kushanda downstream process development kudzikisa huwandu, kukohwa kwehutachiona uye kucheneswa kwakawanda uye matanho ekuchinjana buffer. Panguva imwecheteyo, the autokugadzirwa kwemidziyo yedu recipes inoderedza zvakanyanya kudiwa kwekugadzirisa kwemaoko, kusimbisa kushanda kwevashandisi uye kuderedza mari yebasa. Uyezve, isu tinogonesa iwe kuti uve nepamusoro peprotein clearance uye kudzoreredza kwakanyanya kwemaseru anoshanda ane kushomeka kwekuita kwavo. Inopa mhinduro dzakavimbika dzekusefeta dzakasanganiswa nemazinga ekuyerera uye yakakwirira solute kuchengetwa, edu epamberi maficha anogadzirwa kuti anyatso. downstream kushandiswa kwemavectors. Uyezve, yedu yepamusoro automatic masisitimu anoburitsa a mushandisi-ushamwari ruzivo pane akasiyana masikero ekushanda, kuve nechokwadi chemhedzisiro isina musono uye inowirirana.

Nyaya dzekubudirira uye maapplication

Tichifunga nezvekuoma kweiyo bioprocess, hazvishamisi kuti mutengo unozivikanwa unodhura weizvi biotherapies. At TECNIC, tinoda kubatsira vatengi vedu kuita kuti gene therapy iwedzere uye, saka, inowanikwa kune varwere.. Sezvinoita chero tekinoroji nyowani, mutengo wakadzika nekufamba kwenguva nehunyanzvi hwekuvandudza, uye, nepo isu tisingakwanise kupa ruzivo rwakakwana nekuda kwekuvanzika, kwave nezviitiko zvakawanda apo. TECNICMidziyo yemidziyo yakawedzera kugadzirwa kwemishonga iyi.

Semuenzaniso, mumwe wevatengi vedu ikambani yebiotech iyo parizvino kudzidza kushandiswa kweCRISPR CAS 9 ye exon skipping therapy yeDuchenne muscular dystrophy. Ne "kusvetuka" mamwe genetic code zvikamu zveDNA neCrisp Cas tekinoroji, vanogona kudzikisira kushaya simba kwetsandanyama kweichi chiitiko chehutano. Kune iyo In vivo edit, ivo vari kushandisa mavhairasi mavhairasi akagadzirwa neyedu zvizere automatic bioreactor, eLAB Advance uye, nekuda kwekugadzirisa kwayo kwakasiyana-siyana, vanogona kugadzirisa tsika maererano nezvinodiwa zveyedzo yega yega. Kusvika panguva ino, bioreactor yedu yakavapa simba rekukudziridza nehungwaru hwepamusoro uye kudzora huwandu hukuru hwemiyedzo yakasiyana, ichivagonesa kugadzirisa mamiriro etsika yavo vachishandisa automatic control unit ine kutonga kwakazara pamusoro peakakosha paramita yezvidzidzo zvavo.

Ongorora huwandu hwedu hwakakwana hwemidziyo

Ongorora iyo yepamberi biothecnology yeinotevera-chizvarwa cell uye gene kurapwa (CGTs) ne TECNIC's equipment. Yedu bioreactors yakagadzirirwa kushandiswa kwakasiyana, kuve nechokwadi chekushanda uye kunaka. Tsvaga yedu yakazara yakazara biotechnology mhinduro, kusanganisira Bioreactors, Tangential Flow Filtration masisitimu, nezvimwe. Shanyira mapeji edu e Bioreactors ⇀Tangential Flow Sefa ⇀, uye Contact ⇀ kuti uwedzere information uye kubvunza.