Kuongorora Recombinant Protein Production ne TECNIC'S Biotech Solutions

Munzvimbo ine simba ye biotechnology, recombinant mapuroteni kugadzirwa yava imwe yenzira huru dzekuita gadzira zvigadzirwa zvinodikanwa zveprotein kune huwandu hukuru hwehupenyu-sainzi zvikumbiro, senge tsvakiridzo yekurapa nemishonga.

Munguva yakapfuura, nzira huru yekuwana puroteni chaiyo yaive yekuiparadzanisa kubva kune yakasikwa, iyo yaiwanzoita isingashande uye nguva-con.suming. Nekudaro, nekuda kwekufambira mberi kuchangobva kuitika muinjiniya yemajini uye recombinant molecule biological tekinoroji, iyo biotech landscape yakave neshanduko yekuchinja, zvichiita kuti zvikwanisike ratidza tsika recombinant mapuroteni mukati memaitiro akazvitsaurira ekutaura, akadai sebhakitiriya, mbiriso, masero ezvipembenene, algal system uye masero emammalian.

ichi biotechnological innovation haina kungokosha chete kubiomedicine nemishonga bioprocesses, asiwo kune mamwe maapplication ebiotech, achipa mhinduro nyowani kumatambudziko epasi rose. Sezvo tekinoroji iyi ichiramba ichishanduka, inogona kuvimbisa kuenderera mberi nekushandura maitiro edu kune akawanda minda yesainzi yehupenyu.

At Tecnic isu tinoziva basa rakakosha rinoitwa nemapuroteni anodzokororwa mubiotechnology, uye nechikonzero ichocho, tinoona kuti michina yedu yemazuva ano yakagadzirwa zvine hungwaru kusimudza nekugadzirisa kugadzirwa kwayo kunyatsoshanda, zvakarurama uye scalability. Yedu yakazara huwandu hwe bioreactors inopa simba vaongorori nevagadziri vemaindasitiri kuti vabudirire goho rakawedzerwa, uye masisitimu edu eTFF anobvumira nyore uye automatic kucheneswa yechigadzirwa chaunoda.

Ongorora zvisingaperi zvinobvira ne Tecnic uye usarudze kuti tishande nemumwe wakazvipira kusimudzira mabhindauko mukugadzira zvakanaka kuburikidza nemhinduro dzedu dzekuvandudza.

Chii chinonzi recombinant proteins?

Kana mapuroteni ari iwo horse mubiological system, achityaira akawanda cell bioprocesses, recombinant mapuroteni ndeavo inoratidzwa kubva kune recombinant DNA gene, iyo yave iri kugadzirwa kwemajini kuumbwa kuita an kutaura vector. Yambopinzwa mu host organism, iyo vector inotsigira recombinant protein expression kuburikidza nekunyora uye inotevera RNA dudziro mukati meaida masero ekugamuchira.

The host organism (semuenzaniso E. coli), ikozvino genetically modified, inotova fekitori yakasarudzika ye kugadzirwa kweprotein recombinant. Iyi nzira yebiotechnology inoratidza kukosha kwazvo, ichigonesa kugadzirwa kwemapuroteni ayo angave achinetsa kuwana muhuwandu hukuru kana nemaitiro chaiwo ekugadzirisa uchishandisa nzira dzechinyakare. Recombinant mapuroteni anounza yakakura seti yemabhenefiti kuhupenyu sainzi, sezvo ivo vachibvumira kugadzirwa kwe zvigadzirwa zveprotein zvinodikanwa zvakanyanya zvine goho rakakura muzvigadzirwa zvakakura

Uyezve, by kushandisa genetic engineering nzira kushandura DNA kutevedzana encoding imwe puroteni, zvinogoneka kugadzirisa kuchinja muchimiro chayo, basa, kana zvimwe zvimiro zvechisikigo, kushanda senzira yeku injiniya recombinant mapuroteni ane hunhu hwakarongedzerwa kusangana nezvinodiwa zvakasiyana. Uku kuchinjika kwemaitiro ekugadzirwa kweprotein kunowedzera kupfuura kusikwa kwemapuroteni matsva nezvinangwa zvakasiyana, asi zvakare encom.passndiko kuvandudzwa kwezvinhu zviripo.

Recombinant protein inoshandiswa

Kune akati wandei marudzi emapuroteni anodzokororwa, imwe neimwe iine minda yakasarudzika yekushandiswa, asi mazhinji acho ave akakosha mune dzakasiyana siyana. minda yezvokurapa.

Semuenzaniso, mune indasitiri yemishonga, kugadzirwa kweprotein recombinant kunonyanya kukosha kugadzira majekiseni uye mishonga yekurapa, sezvavanowanzo shanda sechinhu chinoshanda chemishonga mu biopharmaceuticals. Semuyenzaniso, masoja ekudzivirira chirwere akadai semonoclonal antibodies kana immunoglobulin antibodies nedzimwe nzira dzepamusoro dzekurapa dzinowanzo gadzirwa pachishandiswa tsika-inoratidza mapuroteni system. Zvakare, iyo chaiyo uye scalability yerecombinant protein tekinoroji yachinja kuvandudzwa kwezvinodhaka, zvichiita kuti pave nekugadzirwa kwepamusoro-soro, mapuroteni ekurapa akaenzana akadai se insulini, kukura kwezvinhu kana mamwe mahomoni.

Mumunda we zvirwere, sezvo kushanda uye kuwanikwa kweprotein recombinant kunowedzera, ivo vari yakashandiswa kugadzira bvunzo dzakanyanya kunzwisisika uye dzakananga kune zvirwere zvakasiyana-siyana. Semuyenzaniso, kunyangwe hutachiona husingakwanise kusimudzwa, chero kutevedzana kwemajini, zvinokwanisika kukurumidza kupindura kune mavhairasi ari kubuda kana mavhairasi matsva ane ELISA bvunzo inogadzirwa nekushandisa recombinant mapuroteni ehutachiona chaihwo.

 

Basa rinokosha mukutsvakurudza

In tsvakurudzo, dzave dzakakosha kune chidzidzo cheprotein function nekudyidzana, iyo yakakosha pakunzwisisa maitiro ehupenyu uye maitiro ezvirwere. Pamusoro pezvo, nzira dzakaomarara dzakaita sepamusoro plasmon resonance dzinoshandiswa kuongorora kudyidzana kwavo, zvichibatsira mukunzwisisa kwakadzama kwemabasa avo ebhayoloji uye kuwana ruzivo rwekushanda kwemaitiro emapuroteni aya. Semuenzaniso, kuratidza mapuroteni mukati E. coli yakabvumira tsvakiridzo yebiomedical kudzidza yakaoma biochemical nzira uye decipndiye maitiro emolecular ari pasi pemaitiro akasiyana-siyana ehupenyu. Uyezve, kushandisa genome editing maturusi seCRISPR CAS9 kana Cre-lox tekinoroji, kushandiswa kwemapuroteni anodzokororwa emajini akadai seRag 1 neRag 2 kana CNTF gene kwakapa nzwisiso yakakosha mukudzora kwekutaura kwemajini, nzira dzekufambisa chiratidzo, uye kupindirana kwakaoma kunzwisisa pakati pezvikamu zvakasiyana zvemasero.

Uyezve, mapuroteni anodzokororwa anoshandiswa muhunyanzvi tekinoroji haasi akakosha kumushonga chete asiwo kune mamwe minda sekurima uye tekinoroji nyowani yechikafu, inopa mhinduro dzinovimbisa kumatambudziko akaoma muindasitiri yebiotech. Sezvo tekinoroji iyi ichiramba ichishanduka, mukana wayo wezvitsva zveramangwana unovimbisa kuenderera mberi nekushandura maitiro edu kuhupenyu sainzi, zvese mukutsvagisa nekushandiswa kwemaindasitiri, uye TECNIC tinoda kuve chikamu chezvitsva zvebudiriro mhinduro kune shanduka pamwe chete.

Matambudziko mune recombinant protein kugadzira

Iyi biotechnology inoda huwandu hukuru hwezviwanikwa uye kuziva-sei, uye kutadza mukugadzira kunogona kuve nemhedzisiro yakaipa pamwe nekukanganisa kwemari. Zvinetso zvaro zvakajairika encompass nyaya dzine chekuita nekubudirira, kuchena uye scalability uye zvinoda kunyatsotariswa uye nemhinduro dzakasimba dzemibairo yakanaka.

Efficiency optimizing goho uchideredza zviwanikwa

Kuve nechokwadi chekuchena uye kushanda kwekuvimbika

Kugadzirisa mamiriro ezvakatipoteredza kune yakakodzera mapuroteni kutaura

Kukunda scalability matambudziko

Kukosha kweTFF mukugadzirwa kweRecombinant Proteins

Kuti ugadzire yakakwirira-kuchena recombinant mapuroteni ane goho rakakwirira uye mhedzisiro inowirirana, zvakakosha kushanda nemidziyo yakanakisa, kungave uri kugadzira masoja ekudzivirira chirwere ezvinangwa zvekutsvagisa kana kugadzira ma enzymes makuru eindasitiri. Semhinduro yekutanga, TECNIC Bioreactors ⇀ stand out for upstream recombinant protein bioprocessing kugadzirwa, kugadzirisa matambudziko akakosha nehunyanzvi padanho rega rega, kubva pakutsvagisa kusvika pakugadzira indasitiri yakakura.

Titendere kuti tipe kutonga kwakaringana pane ese ezvakatipoteredza paramita, kugadzirisa akanakisa cellular kana hutachiona mamiriro yegoho reprotein uku uchideredza zviwanikwa uye nguva yekushandisa. Yakasimba yekutarisisa kugona kunogonesa kugona kwakanyanya mu bioprocess uye iyo TECNIC automatic control unit ye eLAB, ePILOT uye ePROD ⇀ , iine maindasitiri PLC, inoita kuti iwe ugone kugadzirisa tsika yako mamiriro kuti uwane zvinogoneka uye zvinowirirana mhedzisiro.

Kamwe danho rekupedzisira retsika rawanikwa, chinhu chekutanga kuwana a yakanaka yekupedzisira-chigadzirwa mhedzisiro. The advanced downstream Tangential Flow Sefa ye TECNIC ⇀ zvinonyatso kupatsanura puroteni yakanangwa kubva kune tsvina uye tsvina, kuve nechokwadi chekuchena kwepamusoro nekuyerera kwakanyanya. Zvakare, nekuda kwayo zvizere automamatic system uye akasiyana esarudzo dzekushanda, eLAB TFF ⇀ , ePILOT TFF ⇀ uye ePROD TFF ⇀ batsira pakuchena kwekupedzisira kweiyo bioprocess yako yese.

Pakupedzisira, kupa inherent scalability yemidziyo yedu, TECNIC's bioreactors uye tangential system inochinjika inovimbisa kuvimbika uye kushanda zvakanaka kunodiwa kuzadzisa kudiwa kwekupedzisira kwemhando yepamusoro recombinant mapuroteni. Saka, kunyangwe iwe uri kuita zvekupayona kutsvaga kana kutyaira maindasitiri-chiyero kugadzirwa, TECNICKuzvipira ndiko mhinduro yekushanduka pamwe chete.

Nyaya dzekubudirira uye maapplication

Kunyange isu tisingakwanise kupa ruzivo rwakakwana nekuda kwekuvanzika, kwave kune dzakawanda zviitiko apo TECNICMidziyo yemidziyo yakawedzera zvakanyanya kugadzirwa kwemapuroteni anodzokororwa. Vatengi vedu vakaisa kuvimba kwavo matiri, uye kubudirira kwezvakapihwa TECNIC mhinduro dzinosimbisa kunaka kwedu uye kuzvipira kushanduka pamwe chete.

Semuenzaniso, munharaunda yetsva yekutsvaga chikafu, mumwe wevatengi vedu anovavarira gadzira mazai anoshanda zvakanyanya mapuroteni uchishandisa isiri yemhuka sosi. Kunyange vanga vaine tekinoroji neruzivo, vakasangana nematambudziko akati wandei panguva yekugadzira Scale-Up. Kushandisa the TECNIC eLAB Advanced Bioreactor, vakakwanisa kukwidziridza sisitimu yekutaura, zvichitungamira kune a kuwedzera kukuru mune zvose goho uye kuchena. Zvakare, kuwanda kwechishandiso ichi, pamwe chete neayo scalability maficha, zvakavabvumira kushanda zvisina musono midziyo yakawanda yematanho akasiyana ekugadzira maitiro. Nekuda kweizvozvo, mutengi wedu akazadzisa zvibodzwa zvavo zvekugadzira nekuwedzera kugona uye kunaka kwechigadzirwa, kuwana mhedzisiro yeprojekiti yavo.

Kuzvipira kwedu kumapurojekiti evatengi vedu kunovimbisa kuti isu tinogara tichigadziridzwa pane chero zviitiko kana rumwe rubatsiro rungangodiwa, uye parizvino, isu tichiri kupa rubatsiro runoenderera uye kuramba takabatikana nebasa iri revatengi.

Muchikamu chemishonga, TECNIC inoshandiswa kushandira pamwe nemakambani ari yakatarisana nekugadzirwa kwakakura kwezvigadzirwa zvebiotech pharma zvakaita se monoclonal antibodies ⇀, mishonga yekurwisa cancer, sero kurapwa ⇀, Uye zvakawanda.

Izvi zvekucheka-kumucheto zvemishonga uye biotechnology zvigadzirwa zvinowanzoda kushandiswa kwe bioreactors nokuti upstream process uye downstream tangential filtration systems kuve nechokwadi chegoho rakakwana, kuchena, uye scalability.

Semuyenzaniso, TECNIC inobatanidzwa zvakanyanya mukugadzirwa kukuru kwe innovative therapeutic antibiotic yakagadzirirwa zvipfuyo. Mune iyi chaiyo biotechnological kuedza, yedu ePROD midziyo inoita basa rakakosha, ichipa iko kurongeka uye kutonga kwakakwana kweiyo bioprocess kuve nechokwadi chegoho repamusoro rinotarisirwa purojekiti iyi. Mushure mezvo, yedu ePROD TFF inopatsanura zvinobudirira chinangwa chepeptide kubva kune vezvenhau tsika uye kumwe kusvibiswa. Parizvino, chirongwa chese chinosvika a mwero wepamusoro wekuchena pamwe chete nemitengo yegoho inoshamisa, chirevo chekuzvipira kwedu kune kubudirira kwevatengi nekupa mhinduro dzakakwana padanho rega rega reiyo bioprocess.

Ongorora huwandu hwedu hwakakwana hwemidziyo

Ongorora kumberi kwerecombinant protein nemidziyo yedu yepamusoro. Yedu bioreactors yakagadzirirwa kushandiswa kwakasiyana, kuve nechokwadi chekushanda uye kunaka. Tsvaga yedu yakazara yakazara biotechnology mhinduro, kusanganisira Bioreactors, Tangential Flow Filtration masisitimu, nezvimwe. Shanyira mapeji edu e Bioreactors ⇀, Tangential Flow Sefa ⇀, uye Contact ⇀ kuti uwedzere information uye kubvunza.